Anti-Caspase-14 Monoclonal Antibody for WB, ELISA - P31944

Core Product Specifications and Parameters

Biological Background: Caspase-14 Function and Localization

• Caspase-14 is a non-apoptotic caspase predominantly expressed in the epidermal stratum corneum and involved in terminal keratinocyte differentiation. Related references: PMID:15556625
• It plays a critical role in skin cornification by proteolytically processing filaggrin, a key step in epidermal barrier formation.
• The enzyme recognizes the WEHD tetrapeptide substrate motif and exhibits activity toward synthetic caspase substrates like WEHD-ACF. Related references: PMID:16854378, PMID:19960512
• Caspase-14 participates in DNA degradation in differentiated keratinocytes by cleaving DFFA/ICAD, which releases active DFFB/CAD. Related references: PMID:24743736
• It may contribute to retinal pigment epithelial cell barrier function. Related references: PMID:25121097
• Subcellularly, Caspase-14 localizes to both the cytoplasm and nucleus.
• In human skin, it is expressed in suprabasal keratinocytes of the spinous and granular layers, and its mature p17/p10 and p20/p8 forms are detected in the stratum corneum and upper granular cells.
• Additional keywords highlight it as a thiol protease zymogen linked to ichthyosis, reflecting its importance in skin homeostasis.

Experimental Guidance and Technical Tips

• Consider using this antibody for Western blot and ELISA applications as specified.
• Validate the antibody in relevant sample systems, such as human, mouse, or rat epidermal lysates, ensuring cross-reactivity matches your experimental needs.
• The immunogen corresponds to amino acids 100–200, potentially recognizing both pro and intermediate forms of Caspase-14.
• Due to protein processing, confirm the detected band size (~28 kDa) in your experimental setup, and note that reducing conditions may be beneficial.
• Optimize blocking and antibody dilution conditions for your specific assay to achieve clear, specific signals.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material sourcing partner, CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies in epidermal biology, cornification, and skin-related disorders, this Caspase-14 antibody complements our extensive portfolio of rare-target raw materials, supporting your concept-to-clinic development needs.