Anti-Caspase-12 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6UXS9

Core Product Specifications and Parameters

Biological Background: Inactive Caspase-12 (CASP12) Function and Localization

• Inactive caspase-12 (CASP12) is a member of the caspase family and is widely expressed, with highest levels in the lung.
• May function as a negative regulator of inflammatory responses and innate immunity, reducing cytokine release during bacterial lipopolysaccharide (LPS) infection.
• Reduces activation of NF-kappa-B in response to TNF, as demonstrated in functional studies. Related references: PMID:15129283
• Likely lacks protease activity, distinguishing it from active caspases involved in apoptosis.
• Exists in multiple isoforms due to alternative splicing and is regulated by phosphorylation.
• Localization details are not fully characterized, but given its regulatory role, it may be cytosolic or associated with signaling complexes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300 of human caspase-12. This region may be conserved in mouse and rat isoforms, consistent with the reported cross-reactivity.
• For Western blot, the expected protein weight is approximately 39 kDa. Consider using appropriate positive controls (e.g., lung tissue lysates) where expression is enriched.
• In IHC-P, validate staining with a suitable tissue sample; lung tissue is recommended due to high expression.
• ELISA applications can be developed using this antibody as a capture or detection reagent; optimal working concentrations should be determined empirically.
• Since caspase-12 may exist in multiple splice variants, verify that the detected band corresponds to your target isoform using reference sequences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials and technical support. This Caspase-12 antibody is well-suited for investigations into innate immunity, inflammatory regulation, and NF-kB signaling. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and sourcing for rare or challenging targets to accelerate your assay development from concept to clinic.