Anti-Caspase-1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P29466

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1 is a thiol protease that plays a central role in inflammatory responses by proteolytically cleaving the precursors of interleukin-1 beta (IL1B) and interleukin 18 (IL18) into their active mature forms. Related references: PMID:15326478 PMID:15498465 PMID:1574116
• It also initiates pyroptosis, a pro-inflammatory programmed cell death pathway, by cleaving Gasdermin-D (GSDMD). This cleavage releases the N-terminal domain of GSDMD, which forms pores in the cell membrane. Related references: PMID:26375003 PMID:32051255 PMID:32109412 PMID:32553275
• In response to bacterial infection, Caspase-1 cleaves and activates CASP7, contributing to plasma membrane repair. Related references: PMID:22464733
• During DNA virus infection, Caspase-1 controls antiviral immunity by cleaving and inactivating CGAS. Related references: PMID:28314590
• Caspase-1 is produced as an inactive zymogen and is activated through assembly into an inflammasome complex. The active enzyme cleaves after an Asp residue at position P1. Related references: PMID:7876192
• Subcellularly, Caspase-1 is found in the cytoplasm and associated with the cell membrane.
• Tissue expression is highest in spleen and lung, with detection in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney, and testis. It is not expressed in the brain.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which may regulate its activity.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against a recombinant immunogen spanning amino acids 120-297 of human Caspase-1. It may recognize both the pro-form and cleaved fragments, but validation in the relevant sample system is recommended.
• For Western blot (WB), a band around 45 kDa is expected for the full-length pro-caspase-1; additional lower bands may correspond to processed subunits.
• For immunofluorescence (IF-P), optimize fixation and permeabilization conditions to preserve both cytoplasmic and membrane-associated signals.
• Due to the polyclonal nature, lot-to-lot variability may occur; consistently validate each new lot for your specific application.
• Cross-reactivity with mouse and rat has been reported, but confirm sequence homology in the immunogen region to ensure comparable reactivity across species.
• For ELISA applications, consider using this antibody as a capture or detection reagent in combination with a matched pair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, offering one-stop access to validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For Caspase-1 research, CamelBio can support your inflammation and pyroptosis studies with this rabbit polyclonal antibody, along with technical consultation and supply chain solutions from concept to clinic. Whether you need custom antibody development, large-scale production, or additional critical raw materials, CamelBio is your trusted partner in accelerating assay development.