Anti-Caspase-1 Rabbit Polyclonal Antibody for WB and ELISA - P29452

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1 (ICE) is a thiol protease that initiates pro-inflammatory responses by cleaving cytokine precursors into their active forms. Related references: PMID:21147462
• Upon inflammasome activation, it processes pro-IL1B and pro-IL18, releasing mature cytokines that drive inflammation. Related references: PMID:21147462
• It also cleaves Gasdermin-D (GSDMD), triggering pyroptosis, a programmed lytic cell death pathway. Related references: PMID:32109412
• During bacterial infection, caspase-1 cleaves and activates CASP7 to promote plasma membrane repair; in DNA virus infection, it inactivates CGAS to control antiviral immunity. Related references: PMID:18667412, PMID:22464733, PMID:35705808, PMID:28314590
• The enzyme localizes to the cytoplasm and cell membrane.
• It is highly expressed in spleen and lung, with lower levels in brain, heart, liver, kidney, testis, and skeletal muscle.
• Synthesized as an inactive zymogen, it undergoes autoproteolytic processing to become active; post-translational modifications include methylation, phosphorylation, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and validate detection in spleen or lung lysates as positive controls; the expected band is approximately 46 kDa.
• In ELISA, the antibody may be used as a capture or detection reagent; titrate appropriately and validate in the relevant sample system.
• As a polyclonal antibody, recognize potential batch-to-batch variability; include appropriate controls in each experiment.
• The immunogen sequence (amino acids 200–300) lies within a region conserved in mouse caspase-1; verify cross-reactivity if using samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research institutes, offering one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. For caspase-1-related inflammation and pyroptosis studies, we provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, along with support for rare target raw-material sourcing. Let us streamline your procurement workflow and accelerate your assay development.