Anti-Caspase-1 Rabbit Monoclonal Antibody for WB, ELISA - P29452

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1, also known as Interleukin-1 beta convertase (ICE) or IL-1BC, is a thiol protease that plays a critical initiator role in inflammatory responses and pyroptosis.
• It proteolytically cleaves pro-IL-1β and pro-IL-18 into their active mature cytokines, driving inflammation (PubMed:21147462). It also cleaves Gasdermin-D (GSDMD) to trigger pyroptotic cell death (PubMed:32109412). Related references: PMID:21147462 , PMID:32109412
• Belongs to the caspase family of cysteine proteases; exists as a zymogen that requires processing and assembly into active inflammasome complexes.
• Subcellularly located in the cytoplasm and at the cell membrane.
• Highly expressed in spleen and lung, with lower levels in brain, heart, liver, kidney, testis, and skeletal muscle.
• In response to bacterial infection, caspase-1 also cleaves and activates CASP7, contributing to plasma membrane repair (PubMed:18667412, PubMed:22464733, PubMed:35705808). Related references: PMID:18667412 , PMID:22464733 , PMID:35705808
• During DNA virus infection, it inactivates the cytosolic DNA sensor cGAS by cleavage, modulating antiviral immunity (PubMed:28314590). Related references: PMID:28314590
• Regulated by post-translational modifications including methylation, phosphorylation, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 119-296 of mouse CASP1, a region that may be well-conserved and accessible; consider its suitability for detecting both pro-form and cleaved active subunits in your specific assay.
• For Western blot, the predicted molecular weight is 46 kDa for the full-length pro-caspase-1; validate the antibody’s ability to detect cleaved fragments by including appropriate positive controls (e.g., inflammasome-activated lysates).
• ELISA application is reported; perform titration and validation in the mouse sample matrices of interest, and ensure specific detection of caspase-1 over other caspase family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical services—from concept to clinic. This Caspase-1 antibody empowers the study of inflammation, pyroptosis, and immune signaling pathways, and can be integrated into immunoassay development. Beyond antibodies, CamelBio offers validated antibody pairs, optimized recombinant proteins, and rare target sourcing to streamline your entire assay workflow.