Anti-Caspase-1 Polyclonal Antibody for WB, IF-P, ELISA - P29466

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1, also known as interleukin-1 beta convertase (ICE) or p45, is a thiol protease and a key member of the cysteine-aspartic acid protease (caspase) family.
• Upon activation within the inflammasome complex, it proteolytically cleaves pro-IL-1β and pro-IL-18 into their active mature forms, initiating pro-inflammatory responses. Related references: PMID:15326478 PMID:15498465 PMID:1574116
• It cleaves gasdermin-D (GSDMD) to trigger pyroptotic cell death, with substrate recognition dependent on an exosite interface rather than a strict consensus cleavage site. Related references: PMID:26375003 PMID:32051255 PMID:32109412 PMID:32553275
• Additional substrates include CASP7, which is cleaved to promote plasma membrane repair during bacterial infection; CGAS, which is inactivated during DNA virus infection; and SPHK2, which is released in apoptotic cells. Related references: PMID:22464733 PMID:28314590 PMID:20197547
• It is primarily localized in the cytoplasm and associates with the cell membrane.
• Caspase-1 is highly expressed in spleen and lung, with lower levels in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney, and testis; it is absent in brain.
• The protein undergoes autoproteolytic maturation from a 45 kDa zymogen, and is subject to phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation. Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the p20 subunit of human pro-caspase-1, suggesting the antibody can recognize both the full-length 45 kDa proenzyme and its processed fragments. In Western blot, consider probing lysates from cells treated with inflammasome activators (e.g., LPS/ATP) to observe caspase-1 activation.
• For immunofluorescence (IF-P), validate the antibody in cell lines or tissues with known CASP1 expression (e.g., THP-1 macrophages, spleen), and optimize permeabilization to detect cytoplasmic and membrane-localized pools.
• In ELISA, use the antibody as a capture or detection reagent for caspase-1 quantification; confirm reactivity against recombinant human CASP1 and cross-reactivity with mouse/rat homologs in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with ready access to premium IVD raw materials, including anti-Caspase-1 antibodies like this polyclonal reagent for inflammasome and pyroptosis studies. Beyond individual antibodies, we offer validated antibody pairs, recombinant proteins, bulk ancillary reagents, and customized sourcing of rare targets to support your assay development from concept to clinic.