Anti-Caspase-1 Monoclonal Antibody for WB, ELISA - P29466

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1, also known as interleukin-1 beta convertase (ICE/p45/IL1BC), is a member of the caspase family of cysteine-aspartic acid proteases (thiol proteases) that plays a central role in inflammatory responses and programmed cell death.
• It is essential for the processing and secretion of the pro-inflammatory cytokines interleukin-1 beta (IL-1β) and interleukin-18 (IL-18) by cleaving their precursor forms into biologically active mature peptides.
  Related references: PMID:15326478, PMID:15498465, PMID:1574116
• Caspase-1 initiates pyroptosis, a lytic form of programmed cell death, through cleavage of gasdermin-D (GSDMD), with substrate recognition involving an exosite interface distinct from the canonical cleavage site.
  Related references: PMID:26375003, PMID:32051255, PMID:32109412, PMID:32553275
• Beyond IL-1β/IL-18 and GSDMD, Caspase-1 also cleaves other substrates including caspase-7 (CASP7) during bacterial infection to promote membrane repair, cyclic GMP-AMP synthase (CGAS) to regulate antiviral immunity, and sphingosine kinase 2 (SPHK2) in apoptotic cells.
  Related references: PMID:22464733, PMID:28314590, PMID:20197547
• It is primarily localized in the cytoplasm but can also associate with the cell membrane.
• Tissue expression is highest in spleen and lung, with lower levels detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney, and testis; it is not expressed in the brain.
• Alternative splicing generates isoforms that are inactive in apoptosis, and the protein is subject to post-translational modifications including phosphorylation, ubiquitin-like conjugation, and processing from a zymogen to active enzyme.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 120–297 of human Caspase-1 (NP_150634.1), which lies within the p20 subunit of the processed enzyme; this region may yield antibodies that recognize both the proform and cleaved fragments, but users should validate in their specific sample matrix.
• For Western blotting, the full-length Caspase-1 proenzyme migrates at approximately 45 kDa; additional bands corresponding to processed p20 and p10 subunits may appear depending on activation state—optimize loading and detection conditions accordingly.
• In ELISA applications, consider using the recombinant immunogen as a reference standard or coating antigen, and verify cross-reactivity with related caspase family members if specificity is critical.
• Cross-reactivity has been confirmed for human; for experiments involving other species, perform independent validation as sequence homology varies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic developers and research labs from concept to clinic with a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. This anti-Caspase-1 monoclonal antibody empowers research into inflammasome biology, inflammatory signaling, and pyroptosis, ideal for developing immunoassays targeting key inflammatory biomarkers. Beyond this product, CamelBio facilitates procurement of bulk monoclonals, rare target reagents, and custom technical services to streamline your development workflow.