Anti-Caspase-1 Monoclonal Antibody for WB, ELISA - P29452

Core Product Specifications and Parameters

Biological Background: Caspase-1 Function and Localization

• Caspase-1 is a thiol protease that plays a critical role in inflammatory responses by proteolytically cleaving the precursors of interleukin-1 beta (IL1B) and interleukin 18 (IL18) into their mature active forms (PubMed:21147462, PubMed:32109412).
  Related references: PMID:21147462, PMID:32109412
• It also initiates pyroptosis, a lytic cell death pathway, by cleaving Gasdermin-D (GSDMD) (PubMed:32109412).
  Related references: PMID:32109412
• After inflammasome activation, caspase-1 cleaves and activates CASP7 to promote plasma membrane repair during bacterial infection (PubMed:18667412, PubMed:22464733, PubMed:35705808).
  Related references: PMID:18667412, PMID:22464733, PMID:35705808
• During DNA virus infection, it controls antiviral immunity by cleaving and inactivating cyclic GMP-AMP synthase (CGAS) (PubMed:28314590).
  Related references: PMID:28314590
• In apoptotic cells, caspase-1 cleaves sphingosine kinase 2 (SPHK2), which is released extracellularly and remains enzymatically active (By similarity).
• Subcellular localization: found in the cytoplasm and associated with the cell membrane.
• Tissue expression: highly expressed in spleen and lung, with lower levels in brain, heart, liver, kidney, testis, and skeletal muscle.
• Known aliases include Interleukin-1 beta convertase (ICE), Interleukin-1 beta-converting enzyme, IL-1BC, and p45.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein, suggesting the antibody likely recognizes the native form of caspase-1; consider validating in mouse-derived samples due to its specific cross-reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 46 kDa (full-length precursor); activated fragments may also be observed. Use appropriate positive controls such as LPS-stimulated mouse macrophages.
• In ELISA applications, pair this antibody with a suitable detection antibody and optimize assay conditions using recombinant caspase-1 protein or cell lysates.
• As a rat monoclonal antibody, ensure secondary detection reagents are compatible; if using on mouse tissues, be aware of potential endogenous IgG interference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive range of products and expert technical support to accelerate your diagnostic development from concept to clinical application. This anti-Caspase-1 rat monoclonal antibody supports research in inflammasome biology and pyroptosis, and can be integrated into custom immunoassay solutions. In addition to validated monoclonal antibodies, CamelBio supplies bulk ancillary reagents, optimized antibody pairs, and assists in sourcing rare-target raw materials to meet your specific procurement needs.