Anti-CASK Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O14936

Core Product Specifications and Parameters

Biological Background: CASK Function and Localization

• CASK is a peripheral plasma membrane protein belonging to the membrane-associated guanylate kinase (MAGUK) family, also referred to as calcium/calmodulin-dependent serine protein kinase or protein lin-2 homolog.
• Functions as a multidomain scaffolding Mg²⁺-independent serine/threonine protein kinase that phosphorylates substrates such as NRXN1, contributing to synaptic transmembrane protein anchoring and ion channel trafficking. Related references: PMID:18423203
• Plays a role in neural development and regulation of gene expression through interaction with the transcription factor TBR1, and associates with the LIN-10-LIN-2-LIN-7 complex to transport NMDA receptor subunit NR2B via KIF17 along microtubules.
• Binds to various cell-surface proteins including amyloid precursor protein, neurexins, and syndecans, and may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and spectrin-binding protein 4.1.
• Localizes to the nucleus, cytoplasm, and cell membrane, reflecting its versatile scaffolding and signaling roles.
• Ubiquitously expressed, with significantly higher levels in brain compared to kidney, lung, and liver, and in fetal brain and kidney relative to fetal lung and liver.
• Contains a SH3 domain, multiple protein‑protein interaction modules, and is subject to alternative splicing; phosphorylated on multiple residues as identified by proteomics approaches.
• Genetic variants in CASK are associated with intellectual disability and neurological disorders, underscoring its critical role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1–300) of human CASK; consider using this antibody to detect full‑length CASK and possibly some isoforms but be aware of potential epitope masking in fixed samples.
• For western blotting, anticipate a band around 105 kDa; we recommend including appropriate positive and negative lysates to confirm specificity, as well as verifying lot‑to‑lot consistency for quantitative comparisons.
• In immunofluorescence/ICC, CASK localization can appear nuclear, cytoplasmic, and membrane‑associated; optimal fixation and permeabilization conditions should be determined empirically for the cell type under study.
• For ELISA, test different coating buffers and antibody dilutions; use a recombinant CASK protein standard to establish a reliable standard curve.
• Because this is a polyclonal reagent, perform routine titration and blocking optimization to reduce non‑specific background in each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers, research laboratories, and academic institutes with a one‑stop supply of IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This CASK polyclonal antibody is part of a comprehensive portfolio targeting synaptic and neurological research; beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and specialized sourcing for rare targets. Partner with CamelBio to streamline your assay development and secure reliable, high‑quality raw materials tailored to your diagnostic pipeline.