Anti-Casein Kinase 2 alpha (CSNK2A1) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P68400

Core Product Specifications and Parameters

Biological Background: Casein Kinase 2 alpha Function and Localization

• CSNK2A1 encodes the catalytic subunit alpha of casein kinase II (CK2), a constitutively active serine/threonine kinase that phosphorylates substrates with acidic residues C‑terminal to the target Ser/Thr. Related references: PMID:11239457, PMID:11704824, PMID:16193064
• During mitosis, CSNK2A1 functions in the p53/TP53‑dependent spindle assembly checkpoint (SAC), maintaining cyclin‑B‑CDK1 activity and G2 arrest upon spindle damage. Related references: PMID:11704824, PMID:19188443
• It regulates apoptosis bidirectionally: it mediates p53/TP53‑dependent apoptosis by phosphorylating p53 at Ser‑392 after UV irradiation, and can also inhibit apoptosis by phosphorylating caspases (CASP9, CASP2) and NOL3, protecting cells from death. Related references: PMID:11239457, PMID:16193064, PMID:22184066
• In the DNA damage response, CSNK2A1 phosphorylates multiple repair proteins (MDC1, MRE11, RAD9A, RAD51, HTATSF1), facilitating their recruitment to damage sites. Related references: PMID:18411307, PMID:18583988, PMID:18678890
• CSNK2A1 directly phosphorylates RNA polymerases I–IV and numerous transcription factors (e.g., NF‑κB, STAT1, CREB1, JUN, FOS, MYC, MYB), thereby exerting broad control over transcription. Related references: PMID:12631575, PMID:19387550, PMID:19387551
• The kinase participates in Wnt signaling by phosphorylating β‑catenin (CTNNB1) and the transcription factor LEF1. Related references: PMID:19387549
• Subcellular localization is predominantly nuclear, consistent with its roles in transcription, DNA repair, and cell cycle control.
• Protein expression is detected in gastric carcinoma tissue, with levels rising as the carcinoma progresses; mutations in CSNK2A1 are linked to the intellectual disability disorder OCNDS.

Experimental Guidance and Technical Tips

• For Western blotting, use a positive control lysate from human, mouse, or rat cells and expect a major band near 45 kDa.
• For IHC‑P, standard antigen retrieval protocols may be applied, but optimal conditions should be determined empirically.
• Since the immunogen spans the full‑length canonical sequence (aa 1‑391), the antibody may recognize multiple isoforms; validate specificity in your target tissue or cell system.
• In ELISA, titrate the antibody and antigen to establish a suitable working range.
• For IF/ICC, fixation and permeabilization conditions must be optimized for each cell type.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop partner for IVD raw‑material sourcing, supporting diagnostic manufacturers and research laboratories worldwide. This anti‑CSNK2A1 polyclonal antibody exemplifies our portfolio of reagents for kinase signaling, cell cycle, and apoptosis research. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and customized sourcing for rare targets—streamlining your path from concept to clinic.