Anti-Casein Kinase 2 alpha (CSNK2A1) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P68400

Core Product Specifications and Parameters

Biological Background: Casein Kinase 2 alpha Function and Localization

• Catalytic subunit of the constitutively active serine/threonine-protein kinase CK2, which phosphorylates numerous substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Related references: PMID:11239457, PMID:11704824, PMID:16193064
• Regulates broad cellular processes including cell cycle progression, apoptosis, transcription, and viral infection, acting as a regulatory node integrating multiple signals. Related references: PMID:12631575, PMID:19387551, PMID:19387552
• During mitosis, functions in the p53/TP53-dependent spindle assembly checkpoint (SAC), maintaining G2 arrest in response to spindle damage. Related references: PMID:11704824, PMID:19188443
• Phosphorylates DNA repair proteins (e.g., MDC1, MRE11, RAD9A, RAD51) to facilitate their recruitment to DNA damage sites. Related references: PMID:18411307, PMID:18583988, PMID:18678890
• Negatively regulates apoptosis by phosphorylating caspases (CASP9, CASP2) and NOL3, inhibiting their activation. Related references: PMID:16193064, PMID:22184066
• Modulates transcription by phosphorylating RNA polymerases I–IV and numerous transcription factors (e.g., NF-kappa-B, STAT1, CREB1). Related references: PMID:12631575, PMID:19387550, PMID:19387551
• Regulates Wnt signaling through phosphorylation of CTNNB1 and LEF1. Related references: PMID:19387549
• Localized predominantly in the nucleus; expressed in gastric carcinoma tissue with increasing levels correlating with progression.
• Annotated with keywords including alternative splicing, apoptosis, ATP-binding, biological rhythms, cell cycle, intellectual disability, kinase, nucleotide-binding, phosphoprotein, serine/threonine-protein kinase, transcription, and Wnt signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human CSNK2A1 (amino acids 1–391), suggesting broad epitope coverage. Consider validating reactivity in your specific experimental system.
• For Western blot, an expected band near 45 kDa is consistent with the calculated molecular weight. Optimize loading controls and antibody dilution using known positive and negative cell lines (e.g., HeLa, HEK293).
• For immunohistochemistry on paraffin-embedded sections, antigen retrieval and primary antibody titration may be necessary to achieve optimal signal. Test cross-reactivity in relevant tissues from human, mouse, or rat.
• ELISA performance should be confirmed using recombinant or native CSNK2A1 protein, with background subtraction and appropriate standard curves.
• The antibody's polyclonal nature may result in lot-to-lot variation; revalidate critical parameters when using new lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering a one-stop supply of IVD raw materials, technical support, and consulting from development to clinical application. This anti-CSNK2A1 polyclonal antibody supports studies in cell signaling, cancer biology, and transcriptional regulation, complementing our portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. From rare target sourcing to large-scale production, CamelBio streamlines your procurement with reliable, high-quality materials tailored to your assay and manufacturing needs.