Anti-Casein Kinase 1 alpha (CSNK1A1) Polyclonal Antibody for WB - P48729

Core Product Specifications and Parameters

Biological Background: Casein Kinase 1 alpha (CSNK1A1) Function and Localization

• Casein kinase I isoform alpha (CSNK1A1, also known as CK1) is a serine/threonine-protein kinase that preferentially phosphorylates acidic substrates such as caseins. Related references: PMID:11955436, PMID:1409656, PMID:18305108
• It participates in the canonical Wnt signaling pathway by phosphorylating beta-catenin (CTNNB1) at Ser-45, thereby regulating its stability and downstream transcriptional activity. Related references: PMID:11955436
• CSNK1A1 may phosphorylate circadian clock proteins PER1 and PER2, contributing to circadian rhythm regulation (By similarity).
• It plays a role in mitotic chromosome segregation, localizing to the centrosome, spindle, and kinetochore during cell division. Related references: PMID:1409656
• The kinase also regulates cytoskeletal dynamics: it mediates keratin cytoskeleton disassembly, which may facilitate epithelial cell migration. Related references: PMID:23902688
• CSNK1A1 positively regulates mTORC1 and mTORC2 signaling by phosphorylating the DEPTOR inhibitor in response to nutrient availability. Related references: PMID:22017875, PMID:22017877
• It acts as a negative regulator of the NLRP3 inflammasome by phosphorylating NLRP3, thus inhibiting inflammasome assembly (By similarity).
• Subcellularly, CSNK1A1 is found in multiple compartments including the cytoplasm, centrosome, kinetochore, nuclear speckles, basal body of cilia, and mitotic spindle.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from amino acids 238-337 of human CSNK1A1 (NP_001883.4), which may confer isoform-specific recognition of CK1α.
• For Western blot (WB) analysis, consider using reducing conditions and a molecular weight marker; the expected band is approximately 39 kDa.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate antigen retrieval and fixation conditions should be optimized; validate the antibody in the relevant cell type or tissue.
• For ELISA, determine optimal coating antigen and antibody dilution empirically to achieve robust signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, offering a broad portfolio of validated antibodies, antigens, and ancillary reagents to support diagnostic kit development and research. For projects targeting cell signaling pathways, Wnt biology, or circadian rhythm mechanisms, CamelBio can supply high-quality polyclonal antibodies like this anti-CSNK1A1 antibody, along with complementary proteins and bulk reagents. With a commitment to one-stop sourcing from concept to clinic, CamelBio enables streamlined procurement and technical consultation for your IVD development needs.