Anti-Casein Kinase 1 alpha (CSNK1A1) Monoclonal Antibody for WB, IHC-P, ELISA - P48729

Core Product Specifications and Parameters

Biological Background: Casein Kinase 1 alpha Function and Localization

• CSNK1A1 (Casein kinase I isoform alpha, CK1) is a serine/threonine-protein kinase belonging to the casein kinase 1 family.
• It preferentially phosphorylates acidic proteins like caseins and has broad substrate specificity.
  Related references: PMID:11955436, PMID:1409656, PMID:18305108
• Participates in the Wnt signaling pathway by phosphorylating beta-catenin (CTNNB1) at Ser-45.
  Related references: PMID:11955436
• Plays a role in chromosome segregation during mitosis.
  Related references: PMID:1409656
• Acts as a positive regulator of mTORC1 and mTORC2 signaling by phosphorylating the DEPTOR inhibitor.
  Related references: PMID:22017875, PMID:22017877
• Localizes to multiple subcellular compartments including cytoplasm, centrosome, kinetochore, nuclear speckles, spindle, and cilium basal body.
• May regulate keratin cytoskeleton disassembly and thereby epithelial cell migration.
  Related references: PMID:23902688
• Acts as an inhibitor of NLRP3 inflammasome assembly by mediating NLRP3 phosphorylation (By similarity).

Experimental Guidance and Technical Tips

• For Western blotting, a band around 39 kDa is expected; verify in relevant cell lines (e.g., HeLa, HEK293) or tissue lysates from human, mouse, or rat sources.
• The immunogen design (aa 238-337) targets the C-terminal region, which may be suitable for detecting both full-length and certain splice variants; validate in the specific experimental system.
• For immunohistochemistry, consider antigen retrieval and antibody dilution optimization in paraffin-embedded tissues; positive and negative controls are recommended.
• ELISA applications may benefit from using recombinant protein as a standard; assess cross-reactivity with other casein kinase isoforms if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, and this CSNK1A1 monoclonal antibody is part of our portfolio for kinase signaling and cell cycle research. Beyond this product, we supply validated antibody pairs, bulk reagents, and rare target sourcing to support your diagnostic and research workflows.