Anti-Cas9 Rabbit Monoclonal Antibody for WB, ELISA - Q99ZW2

Core Product Specifications and Parameters

Biological Background: Cas9 Function and Localization

• Cas9 (CRISPR-associated endonuclease Cas9/Csn1) is an RNA-guided DNA endonuclease that serves as the effector protein of the type II CRISPR-Cas adaptive immune system in Streptococcus pyogenes.
  Related references: PMID:21455174
• In the CRISPR interference pathway, Cas9 forms a complex with a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA). This dual-RNA guided complex recognizes and cleaves double-stranded DNA targets complementary to the crRNA spacer sequence.
• Target recognition and cleavage require a short protospacer adjacent motif (PAM) sequence (5’-NGG-3’ for SpCas9) immediately downstream of the target site. PAM recognition is essential for catalytic activity and helps distinguish self from non-self DNA.
  Related references: PMID:24476820
• Beyond DNA cleavage, Cas9 also participates in the maturation of crRNAs. Together with tracrRNA and host ribonuclease III, it stabilizes the pre-crRNA:tracrRNA duplex for correct processing, though Cas9 itself has no catalytic role in RNA cleavage.
  Related references: PMID:24270795
• Cas9 is a multi-domain protein with DNA-binding, endonuclease, and exonuclease activities. It is dependent on divalent metal ions such as magnesium or manganese for its nucleolytic functions.
• Structurally characterized at high resolution, Cas9 exhibits conformational flexibility that enables its roles in RNA binding, DNA unwinding, and coordinated cleavage by its two nuclease domains (RuvC and HNH).
• The native molecular weight of Cas9 is approximately 158 kDa, consistent with its large multi-domain architecture.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 450–730 of Streptococcus pyogenes Cas9, a region that includes portions of the RuvC nuclease domain. Consider that the antibody may recognize both native and denatured Cas9 protein.
• For Western blotting, the predicted molecular weight of full-length Cas9 is ~158 kDa; validate reactivity in the relevant sample system (e.g., S. pyogenes lysates or engineered cell lines expressing SpCas9).
• When developing an ELISA, evaluate the antibody’s performance against recombinant Cas9 protein or cell extracts. The indicated cross-reactivity (Human, Other) suggests potential for applications involving human cell lines or tissues expressing exogenous Cas9.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For the rapidly advancing CRISPR-based diagnostics field, CamelBio supplies high-quality monoclonal antibodies like this anti-Cas9 antibody, along with comprehensive raw-material solutions including validated antibody pairs, optimized bulk reagents, and tailored sourcing for rare targets. Our integrated supply model bridges the gap between research innovation and clinical assay development.