Anti-CART Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16568

Core Product Specifications and Parameters

Biological Background: CART Function and Localization

• Cocaine- and amphetamine-regulated transcript protein (CART) is a neuropeptide encoded by the CARTPT gene, also referred to as CART prepropeptide. It is proteolytically processed to generate active peptides.
• Functions as a satiety factor, inhibiting both normal and starvation-induced feeding, and completely blocks the feeding response induced by neuropeptide Y, with regulation mediated by leptin in the hypothalamus.
• Promotes neuronal development and survival in vitro, indicating additional roles beyond appetite control.
• The protein is secreted and primarily localized in hypothalamic neurons, including the arcuate nucleus, median eminence, and paraventricular nucleus.
• Classified as a neuropeptide and neurotransmitter, CART is associated with obesity and metabolic regulation. Post-translational modifications include disulfide bond formation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 1-100 of human CART (NP_004282.1). Investigators considering this antibody for applications not listed should validate its performance in the relevant assay system.
• For Western blot (WB), the predicted molecular weight is approximately 13 kDa; ensure appropriate polyacrylamide gel percentage and transfer conditions. Include positive and negative controls from relevant tissue or cell lysates.
• For immunofluorescence/immunocytochemistry (IF/ICC), confirm target expression in the sample and optimize fixation and permeabilization protocols. A blocking step may reduce non-specific binding.
• In ELISA applications, use recombinant CART protein or synthetic peptide as a standard to establish assay sensitivity and specificity.
• As a polyclonal antibody, lot-to-lot variability is possible; it is advisable to titrate the antibody for each new batch and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, providing one-stop access to high-quality antibodies, antigens, and ancillary reagents from discovery to commercialization. This anti-CART polyclonal antibody supports appetite regulation and obesity research, and CamelBio can further assist with validated antibody pairs, optimized monoclonal/polyclonal options, bulk quantities, and custom sourcing for rare targets. Partner with us to streamline your diagnostic development pipeline.