Anti-CARS (Cysteinyl-tRNA synthetase) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P49589

Core Product Specifications and Parameters

Biological Background: Cysteine--tRNA ligase, cytoplasmic Function and Localization

• CARS (cysteine--tRNA ligase, cytoplasmic) is an aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of cysteine to its cognate tRNA(Cys), ensuring accurate translation of the genetic code.
• Alternative splicing generates multiple isoforms of CARS, which may exhibit distinct expression patterns or functional roles.
• CARS functions as a homodimeric class I aminoacyl-tRNA synthetase and contains conserved motifs for ATP binding and zinc coordination, essential for catalytic activity.
• The enzyme is primarily localized to the cytoplasm, where it participates in protein biosynthesis.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzymatic activity or protein interactions.
• Mutations in CARS1 are associated with disorders such as microcephaly, developmental delay, and dwarfism, highlighting its critical role in neurodevelopment and growth.
• CARS has been implicated as a proto-oncogene, with chromosomal rearrangements potentially contributing to tumorigenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 650-748) of human CARS; researchers may consider verifying antibody specificity by using a blocking peptide or knockdown/knockout models.
• For Western blotting, the predicted molecular weight is ~85 kDa; however, due to potential post-translational modifications or isoform variations, additional bands may be observed.
• In IHC-P, proper antigen retrieval and tissue fixation conditions should be optimized for the specific sample type; both human and rat tissues are expected to show reactivity based on cross-reactivity data.
• For ELISA applications, consider using this antibody as a capture or detection reagent after validating performance in the intended assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CARS clone. Our supply chain capabilities span bulk reagent provision, custom antibody development, and rare target sourcing to accelerate your assay development in areas such as protein translation disorders and cancer research.