Anti-Cardiac Troponin T/TNNT2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P45379

Core Product Specifications and Parameters

Biological Background: Cardiac Troponin T Function and Localization

• Troponin T is the tropomyosin-binding subunit of the troponin complex, which regulates calcium-sensitivity of striated muscle actomyosin ATPase activity.
• Encoded by the TNNT2 gene; alternative splicing generates multiple isoforms with differential expression in the heart (e.g., isoform 6 predominates in normal adult heart, isoform 7 in failing adult heart).
• Predominantly expressed in the heart; in adults, expression is greater in the ventricle than atrium, while in fetal heart the opposite pattern is observed.
• As a component of the cardiac thin filament, it localizes to sarcomeres and plays a direct role in muscle contraction.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords.
• UniProt keywords also highlight its involvement in cardiomyopathy, disease variants, 3D structure determination, and direct protein sequencing.
• The calculated molecular weight is approximately 35.9 kDa, but observed bands range from 31–36 kDa due to isoform diversity and modifications.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the N-terminal to central region (aa 1–288) of human cTnT, enabling detection of multiple isoforms.
• For WB, anticipated band sizes between 31–36 kDa; consider using heart tissue lysates as positive control.
• For IHC-P and IF-P, validate fixation and antigen retrieval conditions; heart muscle sections are recommended as reference samples.
• For ELISA, this antibody may serve as a capture or detection reagent; pairing with an appropriate clone should be evaluated.
• Note documented cross-reactivity with mouse and rat, but always verify in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, expert technical services, and consulting from concept to clinic. This anti-cardiac troponin T antibody supports cardiac biomarker research and assay development. Our offerings include validated antibody pairs, bulk monoclonals, and rare target sourcing to accelerate your diagnostic workflows.