Anti-Cardiac troponin T (TNNT2) Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P45379

Core Product Specifications and Parameters

Biological Background: Cardiac troponin T Function and Localization

• Troponin T serves as the tropomyosin-binding subunit of the troponin complex, which regulates calcium-sensitive actomyosin ATPase activity in striated muscle.
• It is essential for cardiac muscle contraction by anchoring the troponin complex to the actin-tropomyosin thin filament within the sarcomere.
• Predominantly expressed in the heart, with isoform 6 being the major form in normal adult ventricle; fetal hearts express isoforms 1, 7, and 8, while isoform 7 is also upregulated in failing adult hearts.
• Alternative splicing of TNNT2 generates multiple isoforms that play distinct roles in cardiac development and disease states.
• Post-translational modifications including phosphorylation and acetylation modulate its interaction with tropomyosin and other troponin subunits.
• Mutations in TNNT2 are linked to cardiomyopathies such as familial hypertrophic cardiomyopathy (CMH2) and dilated cardiomyopathy (CMD1D), underscoring its critical role in cardiac physiology.
• The protein localizes to the thin filaments of cardiac sarcomeres, with a molecular weight of approximately 31-36 kDa.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide, providing high specificity for cardiac troponin T; validate in your specific sample system to confirm reactivity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimized staining may require antigen retrieval and titration on cardiac tissue sections.
• In ELISA applications, consider pairing with appropriate capture/detection antibodies for sandwich assays; cross-reactivity with mouse and rat enables multispecies analysis.
• Handle and store according to recommendations; shipping on ice bags ensures stability during transit.

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CamelBio offers a one-stop sourcing platform for premium IVD raw materials, including validated antibodies like this cardiac troponin T monoclonal antibody. Whether you need optimized recombinant antibodies, bulk ancillary reagents, or tailored sourcing solutions for cardiac biomarkers, our catalog supports your diagnostic development from concept to clinic. Contact us to explore how we can accelerate your assay pipeline with reliable, high-quality materials.