Anti-Cardiac Troponin T Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P45379

Core Product Specifications and Parameters

Biological Background: Cardiac troponin T Function and Localization

• Troponin T, cardiac muscle (cTnT) is the tropomyosin-binding subunit of the troponin complex, which confers calcium sensitivity to striated muscle actomyosin ATPase activity.
• Encoded by the TNNT2 gene, alternative splicing generates multiple isoforms with distinct expression patterns in fetal and adult heart, as well as in failing hearts (isoforms 1, 6, 7, 8).
• In the adult heart, isoform 6 is predominant, while fetal hearts predominantly express isoforms 1, 7, and 8; isoform 7 is re-expressed in failing adult hearts.
• cTnT is a key diagnostic biomarker for myocardial injury, as its release into circulation indicates cardiac muscle damage.
• Post-translational modifications include phosphorylation and acetylation, which may modulate its interaction with tropomyosin and other troponin subunits.
• Mutations in TNNT2 are associated with cardiomyopathies (CMD1D, CMPD2, LVNC6), underscoring its critical role in cardiac muscle function.
• The protein localizes to the thin filament of cardiac sarcomeres, where it anchors the troponin complex to tropomyosin.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-120) of human cardiac troponin T; when performing western blot (WB) on cardiac tissue lysates, expect bands in the 31–36 kDa range, with possible isoform-dependent variations.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), cardiac muscle sections are suitable positive controls; consider antigen retrieval optimization for paraffin-embedded tissues.
• This antibody detects mouse and rat cardiac troponin T, but cross-reactivity should be confirmed in the experimental system; a species-specific positive control is recommended.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody targeting a distinct epitope may improve assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to validated IVD raw materials and technical support. This anti-cardiac troponin T polyclonal antibody is part of our comprehensive portfolio for cardiovascular biomarker development, including optimized antibody pairs and bulk ancillary reagents. For custom sourcing or rare target needs, our team bridges your concept to clinical application with reliable one-stop service.