Anti-Cardiac Troponin I (TNNI3) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P19429

Core Product Specifications and Parameters

Biological Background: Troponin I, cardiac muscle Function and Localization

• Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
• It is a cardiac muscle-specific protein, encoded by the TNNI3 gene, and is essential for normal cardiac contractile function.
• Mutations in TNNI3 are associated with cardiomyopathies, including dilated cardiomyopathy (CMD) and hypertrophic cardiomyopathy, as indicated by the keywords "Cardiomyopathy" and "Disease variant".
• Post-translational modifications such as phosphorylation and acetylation modulate its function and interactions within the sarcomere (keywords: Phosphoprotein, Acetylation).
• The protein has been characterized by direct protein sequencing and proteomics identification, serving as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 100 amino acids of human cardiac troponin I; for Western blotting, expect a band near 24 kDa. Validate with positive controls such as human heart tissue or cell lines.
• For immunofluorescence (IF-P), optimize fixation and permeabilization protocols, as the target localizes to cytoplasmic thin filaments.
• In ELISA, consider pairing this polyclonal antibody with a monoclonal capture antibody for antigen detection; perform cross-reactivity testing with skeletal muscle troponin I isoforms to confirm specificity.
• Due to polyclonal nature, each lot may vary slightly; recommend routine validation with known samples and internal reference standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, including this anti-cardiac troponin I antibody for diagnostic and research applications. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support cardiac biomarker assay development. Leverage our expertise to bridge the gap from concept to clinic with robust, high-quality materials.