Anti-Cardiac Troponin I (TNNI3) Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P19429

Core Product Specifications and Parameters

Biological Background: Troponin I, cardiac muscle Function and Localization

• Troponin I, cardiac muscle (cTnI) is the inhibitory subunit of the cardiac troponin complex, which regulates calcium-dependent striated muscle contraction.
• It binds to actin and inhibits actomyosin ATPase activity in the absence of calcium; this inhibition is relieved upon calcium binding to troponin C.
• The protein undergoes post-translational modifications including phosphorylation and acetylation, which may modulate its function and interactions.
• Mutations in TNNI3 are associated with cardiomyopathies, as reflected by the keywords "Cardiomyopathy" and "Disease variant".
• Structural studies (keyword: 3D-structure) have provided insights into its interaction with other troponin components.
• As a muscle protein, it is predominantly expressed in cardiac muscle, contributing to the tissue-specific regulation of contraction.
• The protein has a molecular weight of approximately 24 kDa, consistent with proteomics identification data.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 24 kDa; consider using cardiac tissue lysates for validation.
• For immunofluorescence on paraffin sections (IF-P), optimize antigen retrieval and validate in heart tissue sections.
• For ELISA, this antibody may be used in sandwich pairs; validate cross-reactivity with serum samples.
• Because the antibody cross-reacts with mouse and rat, it can be utilized in animal models of cardiac injury.
• The immunogen covers the full-length protein, suggesting multi-epitope recognition; however, verify performance in your specific assay system.
• Consider potential interference from endogenous immunoglobulins in tissue samples and use appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including this anti-cardiac troponin I monoclonal antibody for cardiac biomarker assay development. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting your project from concept to clinic. Let CamelBio be your trusted sourcing bridge in the IVD industry.