Anti-Cardiac Troponin I (TNNI3) Polyclonal Antibody for WB, IP, IF-P, ELISA - P19429

Core Product Specifications and Parameters

Biological Background: Troponin I, cardiac muscle Function and Localization

• Troponin I (TnI) is the inhibitory subunit of the troponin complex, which regulates striated muscle contraction by conferring calcium sensitivity to the actomyosin ATPase activity.
• Cardiac troponin I (cTnI) is specifically expressed in cardiac muscle and is a well-established biomarker for myocardial injury.
• The TNNI3 gene encodes the cardiac isoform, and mutations in this gene are linked to inherited cardiomyopathies such as hypertrophic and restrictive cardiomyopathy.
• As an actin-binding protein, cTnI binds to actin-tropomyosin in the absence of calcium, inhibiting contraction; calcium binding to troponin C relieves this inhibition.
• Post-translational modifications, including phosphorylation and acetylation, modulate its function and are critical in cardiac physiology and disease.
• The protein shares conserved domains with other troponin I isoforms (slow-twitch and fast-twitch skeletal muscle), but the N-terminal extension is unique to the cardiac form and contains several phosphorylation sites.
• cTnI localizes to the sarcomeric thin filament, contributing to the structural integrity and calcium-dependent regulation of cardiac myofibrils.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human cTnI (aa 1-210), suggesting the antibody recognizes the majority of the protein sequence. When used in Western blotting, expect a band around 24 kDa in human, mouse, and rat cardiac tissue lysates.
• For immunoprecipitation (IP), consider using 1–2 µg of antibody per 500 µg of protein lysate and optimize based on the target abundance. Include appropriate controls to confirm specificity.
• In immunofluorescence paraffin-embedded sections (IF-P), a heat-induced antigen retrieval step in citrate buffer (pH 6.0) is often beneficial for exposing epitopes. Test with cardiac muscle tissue sections.
• For ELISA, this antibody may be used as a capture or detection reagent. Pair it with a monoclonal anti-cTnI to develop a sensitive sandwich assay; cross-reactivity with mouse and rat cTnI can be advantageous for preclinical studies.
• Since the antibody cross-reacts with mouse and rat, it is suitable for translational research using common animal models. However, always validate in the relevant species and sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement for cardiac marker development by offering this high-quality anti-cTnI polyclonal antibody, along with complementary validated antigen and antibody pairs, optimized monoclonal options, and bulk ancillary reagents. Whether you are developing ELISAs, lateral flow assays, or other immunoassays for troponin I, CamelBio’s integrated supply chain and technical support help accelerate your project from concept to clinic.