Anti-CARD8 Polyclonal Antibody for WB, ELISA - Q9Y2G2

Core Product Specifications and Parameters

Biological Background: CARD8 Function and Localization

• CARD8 (Caspase recruitment domain-containing protein 8), also known as CARDINAL, TUCAN, NDPP1, DAKAR, DACAR, or NDPP, is a member of the CARD-containing protein family.
• CARD8 acts as an inflammasome sensor, recognizing pathogen/damage signals such as HIV-1 protease activity or the Val-boroPro inhibitor, and mediates inflammasome activation leading to pyroptosis of CD4+ T-cells and macrophages.
  Related references: PMID:11408476, PMID:11821383, PMID:15030775, PMID:32840892, PMID:33542150, PMID:36357533
• The full-length protein undergoes autoproteolytic processing within the FIIND domain, generating N-terminal and C-terminal fragments that remain non-covalently associated in the absence of stimuli.
• Upon pathogen detection, the N-terminal fragment is ubiquitinated and degraded by the proteasome, releasing the C-terminal fragment to form the CARD8 inflammasome, which directly recruits pro-caspase-1 and promotes its activation, resulting in GSDMD cleavage and pyroptosis.
  Related references: PMID:32558991, PMID:32051255, PMID:32840892, PMID:33053349, PMID:34019797
• In addition to its inflammasome role, CARD8 also functions as a negative regulator of NF-kappa-B activation and the NLRP3 inflammasome.
  Related references: PMID:11551959, PMID:12067710, PMID:24517500
• CARD8 is localized in the cytoplasm, nucleus, and assembled inflammasome complexes.
• CARD8 is highly expressed in lung, ovary, testis, and placenta, with lower expression in heart, kidney, and liver; it is also expressed in spleen, lymph node, and bone marrow.
  Related references: PMID:11551959, PMID:11821383
• CARD8 plays a role in host defense against HIV-1: it senses HIV-1 protease activity, leading to clearance of latent virus after viral reactivation, while HIV-1 can evade sensing by maintaining protease inactivity in infected cells.
  Related references: PMID:33542150

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-200 amino acid region, so the antibody likely recognizes the full-length CARD8 and its N-terminal fragment. Consider verifying specificity by analyzing autoproteolytic processing products in cell lysates.
• For western blot, use human cell lines or tissues with confirmed CARD8 expression (e.g., THP-1 macrophages, lung or ovary lysates). The predicted full-length protein is ~61 kDa, but cleavage fragments may also be detected. Include appropriate positive and negative controls.
• For ELISA, the antibody can be employed in sandwich or indirect formats. Pair with a detector antibody against a distinct CARD8 epitope to ensure assay specificity.
• As this polyclonal antibody is validated for human CARD8, cross-reactivity with other species should be tested experimentally. Consider using inflammasome activators (e.g., Val-boroPro) or HIV-1 protease inhibitors in relevant infection models to study CARD8 dynamics.

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CamelBio offers diagnostic manufacturers, research laboratories, and institutes a comprehensive one-stop platform for IVD raw-material sourcing, technical support, and consulting—from early R&D through clinical application. For innate immunity and inflammasome research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-CARD8 pAb, and bulk ancillary reagents to accelerate your pyroptosis and infectious disease assay development. Contact us for customized sourcing of rare targets and integrated procurement solutions to meet your project needs.