Anti-CARD19 Rabbit Polyclonal Antibody for WB, ELISA - Q96LW7

Core Product Specifications and Parameters

Biological Background: CARD19 Function and Localization

• CARD19, also known as BinCARD (Bcl10-interacting CARD protein), is a caspase recruitment domain-containing protein encoded by the CARD19 gene (C9orf89) in humans.
• It functions as an inhibitor of BCL10-induced NF-kappa-B activation, possibly by suppressing BCL10 phosphorylation in a CARD-dependent manner.
• The protein is expressed in multiple tissues, including ovary, testis, placenta, skeletal muscle, kidney, lung, heart, liver, thymus, and brain.
• Subcellularly, CARD19 localizes to the nucleus, endoplasmic reticulum membrane, and mitochondrial membrane, suggesting roles in diverse signaling compartments.
• Key post-translational modifications include phosphorylation and disulfide bonds, with alternative splicing generating isoforms that may have distinct functions.
• Structural features include a transmembrane helix that anchors it to membranes, and it is classified as a reference proteome member.
• The protein has an observed molecular weight of approximately 26 kDa, aligning with its calculated mass of 25.6 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100) of CARD19; validate specificity in the relevant sample system and consider the presence of isoforms.
• For Western blot, expect a band near 26 kDa; test both reducing and non-reducing conditions due to potential disulfide bonds influencing migration.
• In ELISA, optimize coating and blocking buffers for polyclonal antibodies to reduce background and ensure assay sensitivity.
• Cross-reactivity with mouse CARD19 is confirmed; when analyzing mouse tissue, include appropriate controls and consider species-specific epitopes.
• Given the membrane association, enrichment by subcellular fractionation or detergent extraction may improve detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated polyclonal antibodies, antibody pairs, and bulk reagents. As a one-stop partner, we support the procurement of high-quality CARD19 antibodies and ancillary products for NF-kappa-B and apoptosis research. Our end-to-end service, from concept to clinic, ensures reliable access to rare-target raw materials and technical expertise.