Anti-CARD14 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BXL6

Core Product Specifications and Parameters

Biological Background: CARD14 Function and Localization

• Acts as a scaffolding protein that activates NF-κB and p38/JNK MAP kinase pathways by forming a complex with BCL10 and MALT1, which is required for MALT1 proteolytic activity and inflammatory gene expression. Related references: PMID:11278692, PMID:21302310, PMID:27071417
• A splice variant may function as a dominant negative regulator of NF-κB, unable to activate the pathway. Related references: PMID:21302310, PMID:26358359
• Contains card, coiled-coil, and MAGUK domains, linking it to apoptosis and inflammatory signaling.
• Localizes predominantly to the cytoplasm.
• Isoform 1 is expressed in placenta and epidermal keratinocytes; isoform 2 is detected in leukocytes and fetal brain.
• Phosphorylated form is indicated; associated with disease variants, particularly psoriasis.
• May also protect cells from apoptosis by participating in TRAF2/3/6-mediated signaling.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 200–500, which includes the coiled-coil domain; verify that this region is conserved across target species and isoforms of interest.
• For Western blot, the predicted molecular weight is ~113 kDa; use a gel percentage and transfer conditions appropriate for high molecular weight proteins.
• For immunohistochemistry on paraffin-embedded tissues, antigen retrieval may be required; consider testing on known positive tissues such as placenta or skin.
• ELISA protocols may benefit from checkerboard titrations to optimize coating antigen and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, including validated anti-CARD14 antibodies for inflammatory pathway studies. Our portfolio covers monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, supporting projects from concept to clinical application with reliable supply chains.