Anti-CARD11 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BXL7

Core Product Specifications and Parameters

Biological Background: CARD11 Function and Localization

• CARD11 (Caspase recruitment domain-containing protein 11, also known as CARMA1) is an adapter protein critical for adaptive immunity, transducing NF-kB activation downstream of T-cell receptor (TCR) and B-cell receptor (BCR) engagement. Related references: PMID:11278692 PMID:11356195 PMID:12356734
• Upon activation, CARD11 homooligomerizes to form a nucleating helical template, recruiting BCL10 via CARD-CARD interactions, which then promotes BCL10 polymerization and MALT1 recruitment; this CBM complex triggers I-kappa-B kinase (IKK) phosphorylation, leading to NF-kB nuclear translocation and activation of MAP kinase p38 pathways. Related references: PMID:11356195 PMID:24074955
• CARD11 stimulates BCL10 phosphorylation and promotes its linear ubiquitination by targeting BCL10 to the RNF31/HOIP ubiquitin ligase complex, enhancing downstream signaling. Related references: PMID:27777308 PMID:11356195
• In addition to NF-kB, CARD11 activates the TORC1 signaling pathway, linking antigen receptor signaling to metabolic regulation. Related references: PMID:28628108
• CARD11 is localized in the cytoplasm and membrane raft regions, consistent with its role in proximal TCR/BCR signal transduction.
• It is predominantly expressed in peripheral blood leukocytes, thymus, spleen, and liver, and detected in various leukemia and lymphoma cell lines, while absent in certain solid tumor cell lines, reflecting its immune-specific functions.
• Keywords include coiled coil, phosphoprotein, disease variant, and isopeptide bond, indicating structural features and post-translational modifications relevant to its signaling function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (amino acids 985-1154); consider validating specificity using CARD11 knockout or knockdown systems.
• For western blot, the predicted molecular weight is ~133 kDa; optimize transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry, membrane raft localization may require mild permeabilization and co-staining with raft markers.
• For ELISA, the polyclonal nature offers high sensitivity; include appropriate controls to confirm detection of endogenous CARD11.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies in common model organisms; verify reactivity in relevant tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering this anti-CARD11 polyclonal antibody ideal for immunology and oncology research. Our capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—supporting your assay development from concept to clinic.