Anti-CARD11 (CARMA1) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9BXL7

Core Product Specifications and Parameters

Biological Background: CARD11 Function and Localization

• CARD11 is an adapter protein essential for adaptive immunity, transducing NF-κB activation downstream of T-cell receptor (TCR) and B-cell receptor (BCR) engagement. Related references: PMID:11278692, PMID:11356195, PMID:12356734
• It forms a multiprotein complex with BCL10 and MALT1, activating both NF-κB and MAP kinase p38 pathways. Related references: PMID:11356195
• Upon activation, CARD11 homooligomerizes into a helical scaffold, recruiting BCL10 via CARD-CARD interactions to promote BCL10 polymerization and MALT1 recruitment, ultimately leading to IKK phosphorylation and NF-κB release. Related references: PMID:24074955
• CARD11 binding to DPP4 induces T-cell proliferation and NF-κB activation in a CD3-dependent manner. Related references: PMID:17287217
• It promotes linear ubiquitination of BCL10 by targeting it to RNF31/HOIP, amplifying downstream signaling. Related references: PMID:27777308
• CARD11 stimulates BCL10 phosphorylation and activates the TORC1 signaling pathway. Related references: PMID:11356195, PMID:28628108
• CARD11 localizes to the cytoplasm and membrane rafts, facilitating signaling complex assembly at the plasma membrane.
• It is expressed in peripheral blood leukocytes, thymus, spleen, liver, and various leukemia/lymphoma cell lines (e.g., HL-60, K-562, Raji, SW480); mutations in CARD11 are associated with B cell expansion with NF-κB and T cell anergy (BENTA) and severe combined immunodeficiency (IMD11).

Experimental Guidance and Technical Tips

• The immunogen (aa 1055–1154) corresponds to the C-terminal region of human CARD11; verify epitope conservation in mouse and rat for cross-reactivity studies.
• For Western blotting, the predicted molecular weight is ~133 kDa; use an appropriate gel percentage (e.g., 8% SDS-PAGE) and high-molecular-weight markers for optimal resolution.
• When performing immunofluorescence (IF-P) or immunohistochemistry (IHC-P), include permeabilization steps and validate with lymphoid tissue sections where CARD11 is highly expressed.
• For ELISA, consider pairing this monoclonal antibody with a detection antibody targeting a different epitope to enhance assay sensitivity.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency; always include appropriate positive (e.g., Raji cell lysate) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, supporting immunology and cell signaling research with products like this anti-CARD11 monoclonal antibody. We offer validated antibody pairs, optimized recombinant monoclonal antibodies, bulk ancillary reagents, and custom raw-material sourcing to accelerate your diagnostic development from concept to clinic.