Anti-CARD10 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BWT7

Core Product Specifications and Parameters

Biological Background: CARD10 Function and Localization

• Caspase recruitment domain-containing protein 10 (CARD10), also known as CARMA3, BIMP1, or IMD89, is a scaffold protein encoded by the CARD10 gene.
• It plays an important role in mediating the activation of NF-kappa-B, a key transcription factor in immune and inflammatory responses, through BCL10 or EGFR signaling pathways.
• CARD10 is localized to the cytoplasm, where it likely assembles signaling complexes.
• Tissue expression analysis reveals CARD10 protein in adult heart, kidney, and liver, with lower levels in intestine, placenta, muscle, and lung; it is also detected in fetal lung, liver, and kidney.
• The protein contains a caspase recruitment domain (CARD) and predicted coiled-coil regions, and undergoes alternative splicing, which may generate multiple isoforms.
• CARD10 is identified as a phosphoprotein, suggesting regulation by phosphorylation, and is part of the UniProt Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 863-1032 of human CARD10; the antibody is expected to recognize this epitope region in both native and denatured protein forms.
• For Western blotting, consider the predicted molecular weight of approximately 116 kDa, and validate the observed band against a relevant positive control (e.g., lysates from heart or kidney tissue).
• When performing immunohistochemistry (IHC-P), optimize antigen retrieval conditions and antibody dilution using appropriate tissue sections known to express CARD10.
• As a polyclonal antibody, it may exhibit batch-to-batch variation; include appropriate controls and validate in the specific assay system used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering diagnostic manufacturers and research institutes streamlined access to validated antibodies and ancillary reagents. This anti-CARD10 product supports studies on NF-kB signaling, a pathway implicated in inflammation and cancer. Our portfolio includes optimized polyclonal and monoclonal antibodies, bulk reagent supply, and custom sourcing for rare targets, helping accelerate your development from concept to clinic.