Anti-CAR/CXADR Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P78310

Core Product Specifications and Parameters

Biological Background: CAR/CXADR Function and Localization

• CAR/CXADR (Coxsackievirus and adenovirus receptor), also known as CAR, HCVADR, or CAR4/6, is encoded by the CXADR gene in humans (UniProt P78310).
• It is a component of the epithelial apical junction complex, functioning as a homophilic cell adhesion molecule essential for tight junction integrity.
• CAR/CXADR mediates transepithelial migration of leukocytes through interaction with JAML, leading to gamma-delta T-cell activation, proliferation, and cytokine production, which promotes epithelial repair.
• The protein serves as a receptor for adenovirus type C and coxsackievirus B1-B6, facilitating viral entry into host cells.
• CAR/CXADR localizes to the cell membrane, particularly at basolateral membranes, tight junctions, and adherens junctions; it is also found secreted.
• Tissue expression is detected in pancreas, brain, heart, small intestine, testis, and prostate, with lower levels in liver and lung. Isoform 3 is expressed in heart, lung, and pancreas, while isoform 1 is found at neuromuscular junctions and isoform 2 in blood vessels.
• The protein contains immunoglobulin domains, is glycosylated, and undergoes palmitoylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band approximately 40 kDa when probing endogenous CAR/CXADR; verify in a relevant cell or tissue lysate (e.g., epithelial or cardiac tissue).
• In immunofluorescence or immunocytochemistry, optimize fixation and permeabilization conditions, as CAR/CXADR is a transmembrane protein at tight junctions and can also be secreted.
• The antibody is validated for WB, IF/ICC, and ELISA; cross-reactivity with mouse and rat suggests suitability for cross-species studies, but always confirm performance in your experimental system.
• Consider using the antibody in functional studies of tight junction integrity, leukocyte transmigration, or viral receptor assays, but titrate and validate for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined procurement pathway for high-quality IVD raw materials, including validated antibody pairs and monoclonal antibodies like this anti-CAR/CXADR rabbit mAb. With expertise in molecular biology and B2B sourcing, we support cell adhesion and viral entry research by supplying optimized reagents and bulk ancillary materials. Our one-stop model covers everything from concept to clinic, connecting you with rare-target raw materials and technical consulting to accelerate your assay development.