Anti-CAR/CXADR Polyclonal Antibody for WB, ELISA - P78310

Core Product Specifications and Parameters

Biological Background: CAR/CXADR Function and Localization

• Encodes the Coxsackievirus and adenovirus receptor (CAR), also known as CVB3-binding protein or HCVADR; a type I membrane protein with two immunoglobulin-like domains.
• Functions as a homophilic cell adhesion molecule within the epithelial apical junction complex, essential for tight junction integrity and barrier function.
• Mediates transepithelial leukocyte migration by binding to JAML on gamma-delta T-cells, triggering PI3K and MAP kinase signaling that promotes T-cell proliferation and cytokine production for tissue repair.
• Acts as a cellular receptor for adenovirus type C and coxsackievirus B1–B6, facilitating viral entry into host cells.
• Localizes to the plasma membrane, predominantly at basolateral surfaces, tight junctions, and adherens junctions; also exists in secreted isoforms.
• Exhibits tissue-specific expression patterns: high levels in pancreas, brain, heart; moderate in small intestine, testis, prostate; lower in liver and lung; isoform 1 at neuromuscular junctions, isoform 2 in blood vessels.
• Undergoes alternative splicing, disulfide bond formation, N-linked glycosylation, palmitoylation, and phosphorylation; these modifications likely regulate trafficking, adhesion, and signaling.
• Keywords: Immunoglobulin domain, transmembrane helix, signal peptide, host-virus interaction; heart expression elevated in dilated cardiomyopathy.

Experimental Guidance and Technical Tips

• The immunogen spans the entire extracellular domain (aa 20–237), enabling recognition of native, denatured, and possibly glycosylated forms of CAR; therefore, it is suitable for both WB and ELISA applications.
• For Western blot, expect a band near 40 kDa (predicted molecular weight) in human, mouse, and rat tissue lysates; glycosylation may cause slight mobility shifts.
• Validate antibody specificity by using appropriate positive (e.g., heart, pancreas) and negative controls, perhaps with siRNA knockdown or CAR-knockout cell lines.
• For ELISA, coating antigens with the recombinant immunogen or cell lysates may require optimization; due to polyclonal nature, check lot-to-lot consistency.
• Given its role as a viral receptor, this antibody could be used in viral attachment or neutralization assays when paired with functional blocking experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined one-stop supply channel for IVD raw materials. Whether you need validated antibody pairs for immunoassay development, bulk polyclonal antibodies against cell adhesion and viral entry targets like CAR/CXADR, or ancillary reagents, our sourcing capabilities help accelerate your workflow. Reach out to explore custom solutions for rare target reagents.