Anti-Carbonic Anhydrase 9 (CA9/G250) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q16790

Core Product Specifications and Parameters

Biological Background: Carbonic Anhydrase 9 (CA9/G250) Function and Localization

• Carbonic anhydrase 9 (CA9, also known as CA-IX, CAIX, MN, G250) is a member of the carbonic anhydrase family that catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons, playing a key role in pH regulation.
• It is a transmembrane enzyme predominantly localized at the cell membrane and microvillus membrane, but has also been detected in the nucleus and nucleolus.
• Expression is highly restricted in normal tissues, with the most abundant expression found in the epithelial cells of gastric mucosa; however, it is strongly upregulated in many carcinoma cell lines, making it a well-known tumor-associated antigen.
• CA9 is involved in the adaptation of tumor cells to hypoxic and acidic microenvironments, facilitating cancer cell survival and invasion.
• The protein contains a zinc-binding catalytic domain and undergoes several post-translational modifications, including disulfide bond formation, glycosylation, and phosphorylation; its mature form is also subject to proteolytic shedding.
• According to UniProt, the calculated molecular weight is approximately 49.7 kDa, and it migrates at around 50 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide sequence (amino acids 200-300) of human CA9; consider the epitope location (intracellular domain) when designing experiments and selecting permeabilization or antigen retrieval protocols.
• For Western blotting, a main band around 50 kDa is expected; validate the antibody in cell lines with known CA9 expression levels (e.g., carcinoma cell lines) and optimize loading and blocking conditions.
• For immunohistochemistry (IHC-P), perform antigen retrieval using heat-mediated or enzymatic methods, and titrate the antibody to minimize background; include appropriate positive and negative tissue controls.
• In ELISA applications, the antibody can potentially be used as a capture or detection reagent; consider pairing with other CA9-specific antibodies and validate in the relevant sample matrix.
• Always verify specificity using knockout or knockdown models if available, and consider cross-reactivity with other carbonic anhydrase family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories worldwide. This Carbonic Anhydrase 9 (CA9/G250) antibody is well-suited for cancer biomarker studies and assay development, and we offer reliable supply of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects requiring rare target raw-material sourcing or customized development, our integrated technical services and consulting help streamline your path from concept to clinic.