Anti-Carbonic Anhydrase 2 (CA2) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P00918

Core Product Specifications and Parameters

Biological Background: Carbonic Anhydrase 2 (CA2) Function and Localization

• Carbonic anhydrase 2 catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons. Related references: PMID:11327835 | PMID:11802772 | PMID:11831900

• It can also hydrate cyanamide to urea. Related references: PMID:10550681 | PMID:11015219

• CA2 stimulates the chloride-bicarbonate exchange activity of SLC26A6. Related references: PMID:15990874

• Essential for bone resorption and osteoclast differentiation. Related references: PMID:15300855

• Involved in the regulation of fluid secretion into the anterior chamber of the eye.

• Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.

• Subcellularly, CA2 localizes to the cytoplasm and is also associated with the cell membrane.

• Keywords include metal-binding (zinc), acetylation, phosphoprotein, and disease variant osteopetrosis.

Experimental Guidance and Technical Tips

• For Western blot (WB), anticipate a band at approximately 29 kDa; use a positive control lysate from CA2-expressing tissues such as kidney or colon.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval with citrate buffer (pH 6.0) or EDTA buffer, and validate staining in relevant tissue sections.
• The antibody is a rabbit monoclonal; ensure secondary detection reagents are specific for rabbit IgG and compatible with the detection system.
• The immunogen peptide (aa 161-260) corresponds to a region conserved across human, mouse, and rat, supporting the observed cross-reactivity; validation in these species is recommended.
• For ELISA, titrate the antibody appropriately and consider using a recombinant CA2 protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For carbonic anhydrase research and diagnostic development, we offer validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. Whether you need rare target raw-material sourcing or technical support for assay optimization, CamelBio is your trusted partner.