Anti-Carbonic Anhydrase 2 (CA2) Polyclonal Antibody for WB, ELISA - P00918

Core Product Specifications and Parameters

Biological Background: Carbonic Anhydrase 2 Function and Localization

• Carbonic anhydrase 2 (CA2) is a zinc-dependent metalloenzyme belonging to the alpha-carbonic anhydrase family, encoded by the CA2 gene.
• Reversibly catalyzes the hydration of carbon dioxide to bicarbonate and protons, a fundamental reaction for pH homeostasis and ion transport. Related references: PMID:11327835 PMID:11802772 PMID:11831900
• Exhibits cyanamide hydratase activity, converting cyanamide to urea. Related references: PMID:10550681 PMID:11015219
• Stimulates the chloride-bicarbonate exchange activity of the anion exchanger SLC26A6. Related references: PMID:15990874
• Plays an essential role in osteoclast differentiation and bone resorption. Related references: PMID:15300855
• Localized to the cytoplasm and cell membrane, where it participates in intracellular pH regulation, particularly in the duodenal upper villous epithelium during proton-coupled peptide absorption, and in the secretion of fluid into the anterior chamber of the eye.
• Post-translationally modified by acetylation and phosphorylation; requires a zinc ion as a cofactor for enzymatic activity.
• Genetic mutations in CA2 are associated with autosomal recessive osteopetrosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CA2 protein (amino acids 1-250), which may provide robust detection of endogenous CA2 in various sample types.
• For Western blotting, a band near 29 kDa is expected; consider validating detection in relevant human and mouse tissue or cell lysates.
• When performing ELISA, using purified recombinant CA2 as a standard can help establish assay sensitivity and specificity.
• Avoid chelating agents in lysis and assay buffers, as zinc chelation may disrupt CA2 conformation and affect antibody binding.
• As a polyclonal antibody recognizing multiple epitopes, it may be particularly effective for detecting denatured CA2 in Western blot applications.
• Cross-reactivity with mouse suggests potential reactivity with other mammalian species, but validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing for enzymes like carbonic anhydrases. Whether you require optimized polyclonal antibodies for pH homeostasis research or custom reagent development, CamelBio streamlines your supply chain from concept to clinic.