Anti-Carbonic Anhydrase 1 (CA1) Monoclonal Antibody for WB, ELISA - P00915

Core Product Specifications and Parameters

Biological Background: Carbonic Anhydrase 1 (CA1) Function and Localization

• Carbonic anhydrase 1 (CA1) is a member of the alpha-carbonic anhydrase family and is also known as carbonate dehydratase I, carbonic anhydrase B (CAB), and CA-I. It is expressed widely, with high levels in erythrocytes.
• CA1 catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons, a critical reaction in pH regulation, gas exchange, and ion transport.
  Related references:
  PMID:10550681 PMID:16506782 PMID:16686544
• The enzyme can also hydrate cyanamide to urea, indicating substrate versatility.
  Related references:
  PMID:10550681
• CA1 is a zinc metalloenzyme; each monomer binds one zinc ion essential for catalytic activity.
• Subcellularly, CA1 localizes to the cytoplasm, consistent with its role in intracellular pH homeostasis.
• Post-translational modifications include acetylation, which may regulate enzyme stability or activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 160-261) of human CA1, which is conserved; consider sequence alignment to confirm expected cross-reactivity with mouse and rat orthologs.
• For Western blot (WB) analysis, a band at approximately 29 kDa is expected under reducing conditions. Use standard SDS-PAGE and transfer protocols.
• For ELISA, the antibody may be applied as a capture or detection reagent; optimize coating concentrations and blocking conditions for your specific assay format.
• Validate antibody performance in the relevant sample matrix (e.g., human, mouse, rat lysates) and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic developers and researchers from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need carbonic anhydrase-specific detection tools or integrated custom manufacturing solutions, our team ensures reliable supply and technical expertise.