Anti-CAPZB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P47756

Core Product Specifications and Parameters

Biological Background: CAPZB Function and Localization

• CAPZB encodes the beta subunit of the F-actin-capping protein, which binds to the fast-growing (barbed) ends of actin filaments in a Ca²⁺-independent manner, blocking subunit exchange without severing filaments.
• As part of the actin capping protein heterodimer, CAPZB plays a key role in regulating cell morphology and cytoskeletal organization.
• CAPZB is also a component of the dynactin complex, where it caps the barbed ends of actin filaments and stabilizes dynactin structure, contributing to dynein-mediated ultra-processive transport along microtubules.
• The protein localizes to the cytoplasm, associating with the cytoskeleton and concentrating in myofibril sarcomeres of muscle cells.
• CAPZB undergoes alternative splicing, producing multiple isoforms, and is subject to post-translational modifications such as acetylation and phosphorylation.
• The protein has been structurally characterized by 3D structure determination and is a reference proteome component, widely identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 31 kDa; consider loading appropriate positive control lysates from human, mouse, or rat cells.
• In immunofluorescence/ICC, CAPZB shows cytoskeletal and sarcomeric staining; optimize fixation and permeabilization conditions to preserve antigenicity and structure.
• For ELISA applications, the antibody may be used to detect recombinant or native CAPZB; pair with an appropriate secondary antibody and validate sensitivity in the relevant sample matrix.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical assays; consider titration experiments to determine optimal working dilution for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-CAPZB reagent. Covering the cytoskeleton and cell morphology research area, we also supply bulk ancillary reagents and offer rare target raw-material sourcing to support your entire development workflow from concept to clinic.