Anti-CAPZA2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P47755

Core Product Specifications and Parameters

Biological Background: CAPZA2 Function and Localization

• CAPZA2 (F-actin-capping protein subunit alpha-2, also known as CapZ alpha-2) is a key component of the heterodimeric actin capping protein that regulates actin filament dynamics.
• It binds in a calcium-independent manner to the fast-growing (barbed) ends of actin filaments, blocking subunit exchange and stabilizing filament length without severing them.
• The protein is part of the F-actin capping protein family, and alternative splicing generates multiple isoforms (as indicated by the "Alternative splicing" keyword).
• Post-translational modifications include acetylation and phosphorylation, which may modulate its capping activity and interactions (Keywords: Acetylation, Phosphoprotein, Direct protein sequencing).
• The calculated molecular weight from the sequence is approximately 33 kDa (UniProt: 32949 Da), consistent with the observed band in Western blot applications.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human CAPZA2 (amino acids 1–286), suggesting the antibody should recognize most native conformations.
• For Western blot (WB) analysis, a band of approximately 33 kDa is expected; use appropriate loading controls and validate in human and mouse samples.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; consider testing both heat-induced and enzymatic methods.
• For ELISA, the antibody can be used as a detection reagent; titrate concentrations against the target protein.
• Cross-reactivity with other CAPZA isoforms or related capping proteins should be assessed based on sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials. This anti-CAPZA2 antibody supports studies on actin cytoskeleton dynamics and cell motility. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for your assay development pipeline.