Anti-CAPZA1 Rabbit Polyclonal Antibody for WB, ELISA - P52907

Core Product Specifications and Parameters

Biological Background: CAPZA1 Function and Localization

• CAPZA1 encodes F-actin-capping protein subunit alpha-1, also known as CapZ alpha-1, which is a key regulator of actin filament dynamics.
• The protein binds in a Ca2+-independent manner to the barbed ends of actin filaments, blocking subunit exchange without severing filaments.
• CAPZA1 may play a role in the formation of epithelial cell junctions. Related references: PMID:22891260
• Together with the beta subunit (CAPZB), it forms the barbed end of the dynactin complex, stabilizing the complex and activating dynein for transport along microtubules.
• Subcellularly, CAPZA1 is localized to the cytoplasm and cytoskeleton.
• The protein is subject to acetylation and phosphorylation, and it contains structural features determined by X-ray crystallography.

Experimental Guidance and Technical Tips

• As the immunogen covers full-length human CAPZA1 (amino acids 1–286), the antibody is expected to detect endogenous CAPZA1 in whole-cell lysates. For Western blot, consider using a denaturing gel and optimizing antibody dilution in the relevant sample system.
• For ELISA, evaluate direct or indirect detection formats depending on your assay requirements.
• Given its cross-reactivity with human, mouse, and rat, this antibody can be applied across species, but always validate performance in the specific sample context.
• Since CAPZA1 is involved in actin dynamics and cell junctions, the antibody may be useful for studying cytoskeletal reorganization, cell migration, or epithelial integrity.
• The predicted molecular weight is approximately 33 kDa; confirm bands with a molecular weight marker in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable one-stop source for IVD raw materials, including validated antibodies and ancillary reagents for actin cytoskeleton research. For your CAPZA1 detection needs, we offer this polyclonal antibody along with access to custom antibody development, bulk production, and technical support to streamline your assay development from concept to clinic.