Anti-CAPRIN2 Rabbit Polyclonal Antibody for WB, ELISA - Q6IMN6

Core Product Specifications and Parameters

Biological Background: Caprin-2 Function and Localization

• Caprin-2 (also known as EEG-1, C1QDC1, RNG140) is an RNA-binding protein implicated in the regulation of mRNA transport and translation, particularly in neuronal systems.
• It promotes phosphorylation of the Wnt coreceptor LRP6, leading to increased activity of the canonical Wnt signaling pathway (PubMed:18762581). It also facilitates constitutive LRP6 phosphorylation by CDK14/CCNY during the G2/M phase of the cell cycle, potentially priming cells for Wnt responsiveness (PubMed:27821587).

Related references: PMID:18762581 PMID:27821587

• Caprin-2 modulates the expression of proteins involved in synaptic plasticity, likely through its role in mRNA translation control (By similarity).
• It is involved in regulating growth as erythroblasts shift from a highly proliferative state toward terminal differentiation, and may also function in apoptosis (PubMed:14593112).

Related references: PMID:14593112

• Subcellular localization includes the cytoplasm, mitochondrion, and cell membrane, suggesting multifaceted cellular roles.
• Tissue expression is broad, with the highest levels detected in the brain and spleen.
• Multiple isoforms are generated by alternative splicing, and the protein features a coiled-coil domain, calcium- and metal-binding properties, and undergoes phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 88–329 of human CAPRIN2, a region that may be conserved across species; cross-reactivity with human and rat is confirmed.
• For western blotting, a band near 126 kDa is expected; however, due to phosphorylation and alternative splicing, additional or shifted bands may appear under certain conditions.
• When using ELISA, consider pairing with a capture antibody targeting a distinct epitope if developing a sandwich assay, and validate the antibody pair in the relevant sample matrix.
• We recommend including appropriate positive and negative controls (e.g., CAPRIN2-overexpressing lysates or siRNA knockdown) and optimizing detection conditions for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. For researchers focused on Wnt signaling, RNA biology, or cancer, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Contact our team to discuss how we can accelerate your diagnostic or research development with reliable, high-quality reagents.