Anti-CAPN9 Rabbit Polyclonal Antibody for WB, ELISA - O14815

Core Product Specifications and Parameters

Biological Background: CAPN9 Function and Localization

• CAPN9 encodes calpain-9, also known as digestive tract-specific calpain, new calpain 4 (nCL-4), or protein CG36.
• Belongs to the calpain family of calcium-regulated non-lysosomal thiol proteases.
• Functions as a protease requiring calcium; likely involved in intracellular proteolytic processes.
• Expressed predominantly in the stomach, suggesting a specialized role in gastric biology.
• Contains conserved calpain catalytic and calcium-binding domains typical of the family.
• Keywords indicate involvement in proteolysis, calcium binding, metal ion interaction, and alternative splicing.
• The full-length protein has a predicted molecular weight of ~79 kDa (UniProt: 79097 Da).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 391–690, a region that may include part of the calpain catalytic domain; consider sequence alignment to assess isoform coverage.
• Given predominant stomach expression, validate antibody performance in stomach tissue lysates or relevant cell lines for WB.
• Cross-reactivity with mouse and rat is indicated; verify signal specificity in these species if used.
• For ELISA, test recombinant or endogenous CAPN9 in a suitable matrix; optimize coating and blocking conditions.
• Use appropriate positive and negative controls (e.g., CAPN9-overexpressing lysates vs. knockout or low-expressing tissue) to confirm detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pathway for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For research on gastric proteases or calcium-regulated proteolysis, CAPN9 antibody can be sourced alongside other calpain family tools. Their one-stop interface simplifies access to rare-target materials, supporting diagnostic development from concept to clinic.