Anti-CAPN9 Polyclonal Antibody for IHC-P, ELISA - O14815

Core Product Specifications and Parameters

Biological Background: CAPN9 Function and Localization

• CAPN9 encodes calpain-9, a calcium-dependent cysteine protease of the calpain family, also designated nCL-4 (new calpain 4) and digestive tract-specific calpain.
• The protein contains a cysteine protease domain and multiple calcium-binding EF-hand motifs, enabling calcium-regulated proteolytic activity.
• It is classified as a non-lysosomal thiol-protease, participating in limited substrate cleavage implicated in cytoskeletal remodeling and signal transduction.
• Tissue specificity profiling reveals predominant expression in the stomach, suggesting a specialized role in gastric mucosal biology.
• Uniprot keywords indicate alternative splicing produces multiple isoforms, potentially modulating tissue-specific functions.
• The canonical isoform has a predicted molecular weight of approximately 79 kDa, consistent with the observed protein weight.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 391–690 of human CAPN9 (NP_006606.1), covering part of the protease domain and calcium-binding repeats; this recombinant protein may generate antibodies recognizing both native and denatured conformations.
• For IHC-P, consider testing antigen retrieval methods and serial dilutions on human stomach tissue sections, the primary site of CAPN9 expression.
• In ELISA applications, the antibody can be used as a capture or detection reagent; validate performance using recombinant CAPN9 protein or stomach tissue lysates as a positive control.
• As a polyclonal antibody, it may detect multiple isoforms; background can be managed through affinity purification and careful titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CAPN9 polyclonal antibody supports digestive tract and stomach-focused biomarker research. Backed by our extensive supply chain, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development.