Anti-CAPN7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6W3

Core Product Specifications and Parameters

Biological Background: Calpain-7 Function and Localization

• Calpain-7 is a calcium-regulated non-lysosomal thiol protease belonging to the calpain family, functioning as an intracellular cysteine protease with potential roles in nuclear processes.
• The protein is encoded by the CAPN7 gene (also known as PALBH) and is ubiquitously expressed across human tissues, suggesting broad regulatory functions.
• It localizes primarily to the nucleus, indicating involvement in nuclear substrate cleavage or chromatin-associated events.
• Post-translational modifications such as acetylation and phosphorylation have been identified via proteomics, suggesting dynamic regulation of its activity or localization.
• The protein contains repeat regions and a catalytic cysteine residue typical of cysteine proteases, which mediates its proteolytic activity.
• As a calpain family member, it may participate in calcium signaling pathways, though its direct substrates remain to be fully elucidated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 20-220) of human CAPN7. Consider this domain coverage when designing epitope mapping or competition assays.
• For Western blot, a band around 93 kDa is expected. Include appropriate positive controls from human, mouse, or rat lysates and validate band specificity using knockout or knockdown models if available.
• In IF/ICC, nuclear enrichment of the signal should be observed. Optimize fixation and permeabilization conditions for your cell type to preserve nuclear architecture.
• For ELISA applications, the antibody can be tested as a capture or detection reagent. Validate performance with recombinant CAPN7 protein or relevant biological samples under controlled conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to streamline IVD raw material procurement, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For targets like CAPN7 involved in calpain protease pathways, we facilitate sourcing of rare raw materials and provide technical support from concept to clinic. Whether you need consistent lot-to-lot performance or tailored immunoreagents, our platform bridges your development needs efficiently.