Anti-CAPN5 Polyclonal Antibody for WB, IF/ICC, ELISA - O15484

Core Product Specifications and Parameters

Biological Background: CAPN5 Function and Localization

• CAPN5 is a calcium-regulated non-lysosomal thiol protease belonging to the calpain family.
• It is also known as calpain htra-3 and new calpain 3 (nCL-3).
• CAPN5 is widely expressed in many tissues, with prominent expression in the photoreceptor cells of the retina.
• The protein contains a calpain catalytic domain and is involved in calcium-dependent proteolytic cleavage events.
• Mutations in CAPN5 are associated with autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV), a blinding eye disease.
• Structural studies have revealed its 3D conformation, aiding in understanding disease mechanisms.
• Keywords include hydrolase, protease, thiol protease, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 361-640 of human CAPN5; validate antibody reactivity with your target sequence in the relevant species.
• For Western blotting, anticipate a band near 73 kDa; optimize loading and transfer conditions for high molecular weight proteins.
• In immunofluorescence/ICC, consider appropriate fixation and permeabilization methods for your cell type, and include negative controls to confirm specificity.
• For ELISA, the antibody can serve as a capture or detection reagent; optimize coating concentrations and blocking strategies.
• Cross-reactivity with mouse and rat is indicated; confirm by sequence alignment and preliminary experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with premium IVD raw materials, offering validated polyclonal antibodies like anti-CAPN5 to support retinal disease and protease research. Our streamlined supply chain ensures reliable access to bulk reagents, from optimized antibody pairs to ancillary materials, helping you accelerate assay development. Count on CamelBio for end-to-end procurement support, from concept to clinic.