Anti-CAPN3 Polyclonal Antibody for WB, IF/ICC, ELISA - P20807

Core Product Specifications and Parameters

Biological Background: CAPN3 Function and Localization

• Calpain-3 (also known as p94, CANP3, nCL-1) is a muscle-specific member of the calpain family of calcium-dependent cysteine proteases.
• It functions as a calcium-regulated non-lysosomal thiol-protease that proteolytically cleaves CTBP1 at 'His-409' and, together with UTP25, mediates proteasome-independent degradation of p53/TP53. Related references: PMID:23357851 PMID:27657329
• The protein localizes to the cytoplasm, nucleus, and nucleolus, indicating roles in multiple cellular compartments.
• Isoform I is specifically expressed in skeletal muscle, underscoring its tissue-specific physiological function.
• Calpain-3 is associated with limb-girdle muscular dystrophy type 2A (LGMD2A), as reflected in disease variant annotations.
• Key structural features include multiple calcium-binding sites and a thiol protease domain, consistent with its enzymatic activity.
• Alternative splicing generates multiple isoforms, which may have distinct regulatory or functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–150) of human CAPN3, which is conserved in the canonical isoform; it may detect various splice variants containing this region.
• Recommended applications include Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; users should optimize antibody dilution and validate specificity in their experimental system.
• Cross-reactivity with mouse and rat suggests potential utility in rodent models, but performance should be confirmed in relevant tissues (e.g., skeletal muscle).
• Due to the protein's nuclear and cytoplasmic localization, consider appropriate fixation and permeabilization protocols for IF/ICC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. This CAPN3 polyclonal antibody supports research in muscle biology, proteolysis, and limb-girdle muscular dystrophy. CamelBio offers tailored sourcing solutions including validated antibody pairs, bulk reagents, and rare target procurement to accelerate your diagnostic development.