Anti-CAPN10 Polyclonal Antibody for WB, ELISA - Q9HC96

Core Product Specifications and Parameters

Biological Background: CAPN10 Function and Localization

• CAPN10 encodes calpain-10, a calcium-regulated non-lysosomal thiol-protease belonging to the calpain family, which mediates limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
• The protein is detected ubiquitously, with confirmed expression in primary skeletal muscle cells, and may play a critical role in insulin-stimulated glucose uptake, linking it to type 2 diabetes susceptibility.
• Alternative splicing generates multiple isoforms, potentially contributing to tissue-specific regulatory functions.
• Calpain-10 functions as a calcium-dependent cysteine protease, with its activity implicated in cytoskeletal dynamics and intracellular signaling cascades.
• The enzyme is categorized under hydrolases and thiol proteases, highlighting its catalytic mechanism and structural features.
• Genetic variations in CAPN10 have been associated with diabetes mellitus, underscoring its clinical relevance as a biomarker and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-230) of human CAPN10; consider that this may detect multiple isoforms generated by alternative splicing.
• For Western blotting, the predicted molecular weight is around 75 kDa, but observe actual band patterns in endogenous samples due to potential post-translational modifications or isoform variants.
• Validate antibody specificity in relevant human cell lines or tissue lysates known to express CAPN10, such as skeletal muscle or insulin-responsive cells.
• For ELISA applications, optimize coating conditions and test against recombinant CAPN10 protein to assess binding affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop provider for IVD raw materials, offering a wide range of validated antibodies, recombinant proteins, and ancillary reagents. This CAPN10 polyclonal antibody supports diabetes and metabolic disease research, complementing our comprehensive portfolio for diagnostic development. From concept to clinic, CamelBio streamlines your sourcing with reliable, high-quality reagents and expert technical support.