Anti-CAPG Rabbit Polyclonal Antibody (KO Validated) for WB, IHC-P, ELISA - P40121

Core Product Specifications and Parameters

Biological Background: CAPG Function and Localization

• CAPG (macrophage-capping protein), also known as actin regulatory protein CAP-G, MCP, AFCP, HEL-S-66, or PG, is encoded by the CAPG gene (Gene ID: 822) in humans.
• CAPG is a calcium-sensitive actin-binding protein that reversibly caps the barbed ends of actin filaments without severing them, regulating actin dynamics.
• It plays an important role in macrophage function and may regulate cytoplasmic and nuclear structures through interactions with actin.
• CAPG may also bind DNA, suggesting additional nuclear roles.
• The protein localizes to the cytoplasm, nucleus, melanosomes, and cell projections such as lamellipodia and ruffles.
• Tissue specificity is high in macrophages and macrophage-like cells.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.
• Alternative splicing produces multiple isoforms of CAPG.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly full-length human CAPG (aa 1-348), so the polyclonal antibody may recognize multiple isoforms and potentially the mouse and rat orthologs (cross-reactivity confirmed).
• KO validation indicates target specificity; always confirm knockdown efficiency in your experimental system.
• For Western blotting, a band at approximately 38 kDa (UniProt: 38.5 kDa) is expected in human, mouse, and rat samples.
• For IHC-P, optimize antigen retrieval and permeabilization protocols, as CAPG exhibits both nuclear and cytoplasmic localization.
• Consider appropriate controls (e.g., isotype control or KO lysates) for ELISA and other immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For CAPG and actin cytoskeleton-related targets, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials. Our integrated supply chain streamlines your project from concept to clinic.