Anti-CAP1 Rabbit Monoclonal Antibody for WB, ELISA - Q01518

Core Product Specifications and Parameters

Biological Background: CAP1 Function and Localization

• CAP1, also known as adenylyl cyclase-associated protein 1, is an actin-binding protein that directly regulates filament dynamics.
• It is implicated in complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
• The protein is localized to the cell membrane.
• CAP1 belongs to a family of adenylyl cyclase-associated proteins and contains domains for actin binding.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, indicating regulatory potential.
• Alternative splicing generates multiple isoforms of CAP1.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from the N-terminal region (amino acids 1–100) of human CAP1, suggesting specificity for epitopes in this domain.
• In WB, the predicted molecular weight is approximately 52 kDa; consider using appropriate molecular weight markers to confirm band identity.
• Validate the antibody in relevant human sample systems, such as cell lines known to express CAP1 (e.g., HeLa, HEK293).
• For ELISA, consider optimizing coating conditions and testing a dilution series to establish a standard curve for quantitative analysis.
• Due to CAP1's role in actin dynamics, ensure sample preparation methods preserve protein interactions if studying native complexes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CAP1-related research and assay development, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your projects. Whether you need rare target sourcing or bulk raw materials, CamelBio is your trusted partner for streamlining your IVD development pipeline.