Anti-CAP1 Polyclonal Antibody for WB, ELISA - Q01518

Core Product Specifications and Parameters

Biological Background: CAP1 Function and Localization

• CAP1 is adenylyl cyclase-associated protein 1, encoded by the CAP1 gene, and belongs to the CAP family of actin-binding proteins.
• It directly regulates filament dynamics and is implicated in complex developmental processes, including mRNA localization and the establishment of cell polarity.
• CAP1 is localized to the cell membrane, where it may interact with actin and other signaling molecules.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, as identified by proteomics and direct protein sequencing.
• Alternative splicing generates multiple isoforms, and the protein contains isopeptide bonds as indicated by structural studies.
• It is classified under keywords such as actin-binding, cell membrane, and reference proteome, highlighting its role in cytoskeletal regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 1–100 of human CAP1, so the antibody is likely to recognize the N-terminal region. For ELISA, consider using the immunogen peptide or a recombinant protein fragment as the coating antigen.
• For Western blot, a band at approximately 52 kDa is expected in human cell or tissue lysates. Validate the antibody in your sample system, as post-translational modifications or isoform expression may affect the observed molecular weight.
• The antibody is polyclonal and may exhibit batch-to-batch variation; for quantitative applications, titration and consistency checks are recommended.
• No cross-reactivity information with other species is provided; therefore, this antibody is only recommended for human samples unless verified independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials and technical support from concept to clinic. This anti-CAP1 antibody supports research into actin dynamics, cell polarity, and cytoskeletal signaling pathways. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, ensuring you can obtain the right materials for your assay development and manufacturing needs.