Anti-CAND2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75155

Core Product Specifications and Parameters

Biological Background: CAND2 Function and Localization

• CAND2 (Cullin-associated NEDD8-dissociated protein 2), also known as TIP120B, Tp120b, and p120 CAND2, functions as a probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.
• It promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, playing a key role in determining the cellular repertoire of SCF complexes.
• The protein localizes to the nucleus, consistent with its role in ubiquitin ligase complex regulation.
• Tissue specificity: expressed in epididymis (at protein level).
• Associated with the ubiquitin conjugation pathway; additional keywords include Acetylation, Alternative splicing, and potential involvement in Transcription (based on keyword annotation).
• The full-length protein has a calculated molecular weight of approximately 135 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 850–1000 of human CAND2. This C-terminal region may recognize full-length CAND2 and possibly specific isoforms containing this segment.
• For Western blot (WB), the predicted molecular weight is ~135 kDa; consider using an appropriate percentage gel and optimize transfer conditions for high molecular weight proteins.
• For immunohistochemistry (IHC-P), consider validating the antibody in epididymis tissue, where CAND2 expression has been confirmed.
• For ELISA, the antibody can be used as a detection reagent; consider optimizing coating antigen and antibody concentrations for best performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare-target sourcing. This anti-CAND2 antibody supports research into ubiquitin ligase machinery and transcription-related processes. Partner with us to streamline your diagnostic development from concept to clinic.