Anti-CAMP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P51437

Core Product Specifications and Parameters

Biological Background: CAMP (Cathelicidin Antimicrobial Peptide) Function and Localization

• CAMP (Cathelicidin antimicrobial peptide), also known as cathelin-like protein (CLP), is a secreted antimicrobial protein encoded by the Camp gene in mouse.
• It is an integral component of the innate immune system, exerting antimicrobial activity by binding to bacterial lipopolysaccharides (LPS) (By similarity).
• CAMP acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2, contributing to immune cell recruitment.
• The protein is highly expressed in bone marrow and small intestine, with additional expression in testis, spleen, stomach, and intestine; very low expression in heart, lung, and skeletal muscle, and no expression in brain, kidney, or liver.
• Subcellular localization: CAMP is secreted and found in vesicles.
• The precursor protein has a calculated molecular weight of approximately 19.5 kDa, and it contains a signal peptide and a cathelin-like domain.
• Keywords for CAMP include antibiotic, antimicrobial, disulfide bond, immunity, and innate immunity, reflecting its role in host defense.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 73-172 of mouse CAMP, which corresponds to the cathelin-like domain; consider verifying reactivity against the processed antimicrobial peptide in your experimental system.
• For Western blotting, a protein band near 19 kDa (precursor) or lower molecular weight forms (cleaved mature peptide) may be observed; optimize gel percentage and transfer conditions accordingly.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary due to the secreted nature of the protein; validate staining patterns in relevant tissue sections.
• As a polyclonal antibody, lot-to-lot consistency should be assessed and appropriate controls included in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, serving as a single-source partner for diagnostic manufacturers and researchers. This anti-CAMP antibody supports investigations into innate immunity and antimicrobial peptide biology. Beyond antibodies, CamelBio supplies validated antibody pairs, bulk ancillary reagents, and can source rare targets to streamline your assay development from concept to clinic.