Anti-CAMP Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P49913

Core Product Specifications and Parameters

Biological Background: CAMP Function and Localization

• CAMP (cathelicidin antimicrobial peptide) encodes the precursor protein hCAP-18, an integral component of the innate immune system. Related references: PMID:14978112, PMID:16637646, PMID:18818205

• The precursor binds bacterial lipopolysaccharides (LPS) and signals through N-formyl peptide receptors, enhancing chemokine release (e.g., CXCL2). Related references: PMID:16637646, PMID:18818205, PMID:22879591

• Post-translational processing generates various active peptides, notably LL-37, which exhibits broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and viruses. Related references: PMID:14978112, PMID:9736536, PMID:10417311, PMID:8681941

• LL-37 disrupts microbial membranes by forming transmembrane pores, leading to cell lysis. Related references: PMID:22879591, PMID:32753597, PMID:33060695

• LL-37 induces cytokine and chemokine production, including TNF/TNFA and CSF2/GMCSF, in normal human keratinocytes. Related references: PMID:15778390

• The peptide can assemble into chiral supramolecular structures with bacterial quorum-sensing molecules, interfering with biofilm formation. Related references: PMID:34708076

• CAMP is highly expressed in neutrophilic granulocytes and bone marrow; the precursor and its processed forms are secreted and found in vesicles, with extracellular release onto the skin surface to provide innate immune defense.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central cathelin domain (aa 31–170), which is conserved across human and mouse CAMP, supporting the observed cross-reactivity.
• For Western blotting, a starting dilution range of 1:500–1:2000 is typical for polyclonal antibodies; validate using lysates from human neutrophils or HL-60 differentiated cells.
• For immunofluorescence and immunohistochemistry, test antigen retrieval methods and include appropriate controls such as human skin or infected tissue sections.
• In ELISA applications, coat with recombinant CAMP protein or peptide fragments and perform checkerboard titrations to determine optimal antibody and antigen concentrations.
• Always include negative controls (secondary antibody alone) and validate specificity using blocking peptides or knockout models if available.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated supplier for the IVD industry, CamelBio provides one-stop access to high-quality raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and recombinant proteins. Our CAMP antibody supports research in innate immunity and infectious disease, and we also offer custom sourcing for rare targets and bulk ancillary reagents. From concept to clinic, CamelBio is your reliable partner for diagnostic raw material procurement.